This cohort was different from the discovery cohort. The association was tested by us of the four mutations with plasma lipid levels. The techniques used for association and genotyping testing are described in the Supplementary Appendix. APOC3 Genotypes, APOC3 Levels, and CARDIOVASCULAR SYSTEM Disease We following tested the association of APOC3 mutations with coronary heart disease in participants of European, African, and Hispanic ancestry in 15 studies . To investigate the association between APOC3 known level and the chance of coronary heart disease, we used bloodstream samples drawn from 3238 fasting participants in the Framingham Heart Study offspring cohort .20 Plasma APOC3 amounts were measured by using a commercially available immunochemical assay .As a reminder for all those people who want to lose weight and fat, the best diet for them is meals containing low carbohydrates and low saturated extra fat. High proteins foods include; chicken, legumes, lean meat, fish and egg whites. Finally it is very important to note the optimum time to take proteins in a complete day. The optimum time to take them are; you awaken in the morning immediately , last thing before going to bed during the night and immediately after your work out session. 2. CREATINE This is naturally produced by the body in order to supply energy to muscle groups and various nerve cells. Though naturally produced, it could be found in red meat several varieties of fish. Merely put, creatine determines the quantity of energy you shall posses in your muscles.